The original intention of 48-well 1ml tube design is to meet the needs of preserving small frozen tissues. The short and thick 1 ml tube is easier to access tissue than the 2 ml cryopreservation tube. Each tube can put 2-3 tissue blocks of 0.5cm square to meet the needs of one experiment. The low height saves the storage space to the greatest extent. The 48 holes are arranged obliquely, and the spacing between the pipe centers is kept at 18 mm, which coincides with the projection structure center of 96-well of standard SBS structure. In this way, even multi-channel pipetting equipment without spacing adjustment function can be used.